 ผลงานตีพิมพ์ในวารสารวิชาการSeasonality affects post-thaw plasma membrane intactness and sperm velocities in spermatozoa from Thai AI swamp buffaloes (Bubalus bubalis)ผู้แต่ง: Dr.Seri Koonjaenak, Assistant Professor ,  Pongpeng, P, Wirowuthikul, S, Johannisson, A, Kunavongkrit, A, Rodriguez-Martinez, H, วารสาร:   
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ผลงานตีพิมพ์ในวารสารวิชาการSeasonal variation in nuclear DNA integrity of frozen-thawed spermatozoa from Thai AI swamp buffaloes (Bubalus bubalis)ผู้แต่ง:Dr.Seri Koonjaenak, Assistant Professor, Johannisson, A, Pongpeng, P, Wirojwuthikul, S, Kunavongkrit, A, Rodriguez-Martinez, H, วารสาร: |
 การประชุมวิชาการการศึกษาเพื่อเปรียบเทียบสามวิธีสกัดดีเอ็นเอจากน้ำเชื้อโคที่ทำให้ปนเปื้อนเชื้อเลปโตสไปร่าและการวิเคราะห์ผลผลิตพีซีอาร์ด้วยเทคนิคเจล อิเลคโตรโฟรีซิสสองแบบ (2006)ผู้แต่ง:Dr.Ukadej Boonyaprakob, Assistant Professor , Dr.Sirichai Wongnarkpet, Assistant Professor , Mr.Vorasak Patchimasiri, Associate Professor , Mr.Sommai Homswat, Uavechanichkul, R.,, Pongpeng, P., Wirojwuthikul, S., , Suwancharoen, D., การประชุมวิชาการ: |
 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : In Vitro Preservation of Sugarcane Plantlets under Low Temperature ) ผู้เขียน:ดร.เรวัต เลิศฤทัยโยธิน, รองศาสตราจารย์ , Pongpeng Chit-areerat, Rongrong Visessuwan สื่อสิ่งพิมพ์:pdf AbstractPlantlets of two cultivated sugarcane cultivars, Supan 1 and C02, cultured on modified Murashige and Skoog supplemented with 1 mg/I 2,4-dichlorophenoxy acetic acid, 0.1 mg/1 myo-inositol, 0.02mg/1 glycine, 20g/l sucrose and 10% by volume of coconut water (CD media) were used. Plantlets were transferred to incubator controlled at 5?C and 15?C under 24 hours light condition for I and 2 months. After the storage periods, plantlets were cultured under room temperature for I month. Culture under 5?C for 1 month and 15?C for 1 and 2 months could decrease growth of sugarcane plantlets in which all of plantlets could normally rcgrowth under room temperature. Plantlets of sugarcane cultivar Supan I cultured under 5?C for 2 months could. regrowth only 50-60% under room temperature. |
 ที่มา:วารสาร คณะสัตวแพทยศาสตร์หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Quality of Bull Deep Frozen Semen Extended with Different Glycerol Concentrations: Evaluated by Computer Assisted Semen Analysis) ผู้เขียน:Rapiphan Uavechanichkul, Jatuporn Pongpeng, Sinchai Wirojwutthikul, Lertchai Jintapitaksakul, Kunnasant Saikhun, Mukda Rattanapaskorn สื่อสิ่งพิมพ์:pdf AbstractQuality of bull deep frozen semen extended with 87% and 99% glycerol in egg yolk tris extender with one-step and two-step dilution methods assessed by CASA (Computer Assister Semen Analysis) were compared. Semen of 20 Holstein-Fresian bulls aged 3-5 years were collected and frozen once a week for 3 weeks. The result showed the post-thaw sperm motility and progressive motility at 0 h from the 87% and 99% glycerol were not significantly different (P>0.05). The ALH (amplitude of lateral head displacement) and BCF (beat cross frequency) of the sperm from the extender using the 99% glycerol was significantly higher (P<0.05). However, after 5 h of incubation at 37?C the 87% glycerol gave significantly lower motility and progressive motiloty with higher VAP (average path velocity), VCL (curvilinear velocity) and ALH (P<0.05). The results of dilution methods did not differ significantly at 0 h. except at 5 h. the one-step method revealed significant higher motility (P<0.05. There were no effects of glycerol cencentration and dilution methods on post-thaw sperm head elongation, area, plasma membrane and acrosomal integrity (P>0.05). In conclusion, using 99% glycerol and one-step dilution methods gave a better semen quality. |
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